Dual Labelled Probes

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Dual Labelled Probes

We offer a broad spectrum of Probes for Real-Time PCR (TaqMan, Molecular Beacons, etc.) available in our premium grade DNA Reverse Phase HPLC.

Specification for DNA Reverse Phase HPLC (RP-HPLC) Dual Labelled Probes:

  • Deprotected & purified
  • Recommended for methods like PCR, sequencing and hybridization
  • Delivered as solid
  • Modification allowed
  • Certificate of Analysis (CoA) follows
  • 100% Quality Controlled (QC) by MassSpec
  • To be kept frozen at -18 C
  • Average in-house turnover time: 3-5 working days
  • Scale & yield
ProductSynthesis scale (µmol)LengthODEst. no. of PCR reactions
DNA Reverse Phase HPLC0.01n/an/an/a
DNA Reverse Phase HPLC0.048-802200
DNA Reverse Phase HPLC0.208-804400
DNA Reverse Phase HPLC extra long0.2081-1202200
DNA Reverse Phase HPLC1.008-802020.000
Infoirmation

How Dual Labeled Probes Work

A Dual-Labeled Probe is a single-stranded oligonucleotide labeled with two different dyes. A reporter dye is located at the 5’-end and a quencher molecule located at the 3′-end.

The quencher molecule inhibits the natural fluorescence emission of the reporter dye. The primer is elongated by the polymerase and the probe binds to the specific DNA template, hydrolysis releases the reporter dye from the probe/target hybrid, causing an increase of fluorescence. The measured fluorescence signal is directly proportional to the amount of target DNA.

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