PTO
PTO – Phosphothioate Oligonucleotide
Phosphothioate oligonucleotides (PTOs) are modified DNA or RNA molecules in which one sulfur atom replaces an oxygen atom in the internucleotide linkage. This structural modification enhances nuclease resistance and increases stability, making PTOs particularly effective in antisense applications aimed at inhibiting gene expression.
Customizable Options
TAG Copenhagen offers PTO synthesis tailored to meet your specific research needs. Choose from the following configurations:
- Full PTO: Every internucleotide linkage in the oligonucleotide contains a phosphothioate modification, providing maximum stability.
- “Thiocap” PTO: Phosphothioate linkages are selectively incorporated at the 3′ or 5′-end for partial protection.
- Chimera: A hybrid molecule combining stretches of phosphothioate-modified DNA/RNA with unmodified regions.
The level of stability increases with the number of phosphothioate linkages, allowing you to design oligonucleotides that match the demands of your specific applications.

PTO are manufactured to the specifications of our customers
Category
Unmodified PTO
Modified PTO
Plate PTO
Purification method:
Desalted
Reverse Phase Column
RP-HPLC
Scales
40 nmol
200 nmol
1.000 nmol
Delivery Method
Solid
In 200 µL water
Standard Conc. (you add 100 µL water/buffer)
Conc. adjustment (multiple aliquoting and mixing)
Applications of PTO Oligonucleotides
Phosphothioate oligonucleotides are widely used in:
- Molecular Biology Studies: Used in hybridization and other applications requiring robust, stable oligonucleotides.
- Antisense Research: Effective for inhibiting gene expression by targeting complementary RNA sequences.
- Therapeutic Development: Ideal for creating stable nucleic acid-based therapies due to their resistance to enzymatic degradation.
Manufactured to Your Specifications
All PTO oligonucleotides are custom-made according to your precise requirements. Whether you need full PTO coverage, selective modifications, or complex chimera designs, TAG Copenhagen ensures high-quality synthesis tailored to your project.
Let TAG Copenhagen help you harness the stability and versatility of phosphothioate oligonucleotides for your next research breakthrough.
How to Order
Please contact our customer service for a quotation and when ordering Phosphothioate oligonucleotide (PTO).
You can also contact us directly via email or phone for personalized support.
Order by E-mail
Please contact our customer service for a quotation and when ordering Phosphothioate oligonucleotide.